This is an image of a gold crystal taken with the new JEOL JEM 2100F transmission electron microscope. Spacing of the lattice planes in the specially prepared gold crystals is a useful way to test the resolution and image stability of an electron microscope. This is one of the first images taken on our new TEM in Baker Lab at SUNY-ESF. The spacing in the planes of the crystal is approximately 2Å or 0.2 nm. An Angstrom (Å) unit is equal to 0.1 nanometer or 10-10 meters.
As many of you know, we received a National Science Foundation Major Research Instrumentation grant to support SUNY-ESF and partner institutions, Upstate MedicalUniversity and Syracuse University with new electron microscopes. The JEOL JSM IT100LA In Touch Scanning Electron Microscope was installed in January. The installation of the JEOL JEM2100FE transmission electron microscope is in progress.
The Image shows particles of Bermuda pink sand in low vacuum backscattered electron imaging.
JEOL JEM 2100FE Transmission Electron Microscope
200 kV Field Emission Electron Gun
Elemental Analysis with EDS X-ray
Cryo, STEM, Tomography
JEOL JSM IT100LA Scanning Electron Microscope
Elemental Analysis with EDS X-ray
Low Vacuum Imaging
In-situ Freeze Drying
STEM in SEM
Stage Navigation System
Our two new electron microscopes were made possible through a grant from the National Science Foundation. The grant was award to a team of investigators from SUNY-ESF and partner institutions, Syracuse University and Upstate Medical University; Susan Anagnost, Ph.D., N.C. Brown Center Director and Principal Investigator on the grant, Robert P. Smith, M.S., and Ivan Gitsov, Ph.D., of ESF; Stephan Wilkens, Ph.D., of SUNY Upstate Medical University; and Mathew M. Maye, Ph.D., of Syracuse University. Funding for the microscope is also supported by Empire State Development’s Division of Science, Technology and Innovation (NYSTAR) and the three universities.
Electron Microscopy at SUNY-ESF 1955-2017
History of the N. C. Brown Center for Ultrastructure Studies
by Susan Anagnost
The Electron Microscopy Laboratory was established at the SUNY College of Forestry in 1955 as part of the Wood Technology Department, and was the first facility of its kind in Central New York, with the only transmission electron microscope in the area. This lab was established through the efforts of Dr. Wilfred A. Côté.
The N.C. Brown Center for Ultrastructure Studies was established in 1972 with the purchase of the center’s first scanning electron microscope. The purchase was made possible through funds left to the New York State College of Forestry Foundation, Inc. from the estate of Nelson C. Brown. Nelson Cortlandt Brown was a forester and faculty member from 1912-1917, and from 1921-1951 also served as Department Head of the Forestry department.
In addition to providing a unique research facility with initial focus on wood anatomy and ultrastructure, the center began to offer formal graduate level coursework in Light Microscopy and Photomicrography, Transmission Electron Microscopy, Scanning Electron Microscopy, Anatomy and Ultrastructure of Wood, and Interpretation of Cellular Ultrastructure. Today we continue to offer graduate coursework as well as an undergraduate minor in microscopy.
Past and current personnel include Dr. Wilfred A. Côté, Dr. Robert B. Hanna, Dr. Susan E. Anagnost, Arnold Day, John McKeon, Linda Bookman, and Robert P. Smith.
1955 First TEM in Central New York, the RCA EMU-3 1955 3B Transmission Electron Microscope
1957 RCA EMU-3 1957 3F Transmission Electron Microscope
1967 RCA EMU-4A Transmission Electron Microscope
1972 First Scanning Electron Microscope – the ETEC Autoscan SEM
1984 Scanning Electron Microscope ETEC SEM with WDS X-ray
1986 Purchase of the JEOL 2000-EX Transmission Electron Microscope
1997 NSF grant awarded for a Scanning Electron Microscope, the JEOL JSM 5800LV
2015 National Science Foundation grant awarded for a Field Emission TEM and a new SEM
2017 Installation of the JEOL JEM-2100F Transmission Electron Microscope and the JEOL JSM IT100LA Scanning Electron Microscope with funding from NSF, NYSTAR, ESF, UMU and SU
The image in our blog heading is a mosaic of wood “cubes” prepared by Mr. Arnold Day back in the 1970’s. These cubes were painstakingly prepared by sectioning with a sliding microtome the three surfaces; radial, tangential and cross section. Images were then acquired with the ETEC scanning electron microscope. These were featured on the cover of the book, Wood Structure and Identification by H. A. Core, W. A. Côté and A. C. Day, Syracuse University Press, 1979.
The species from left to right, top row: Red Maple 20X, Western Red Cedar 10X, Red Maple 10X, Yellow Birch 50X, Red Maple 10X; bottom row: Yellow Birch 30X, Red Gum 50X, American Elm 10X, Red Gum 10X, Douglas-fir 50X. (X magnification when acquired on the SEM).